Engineering Biology Objectives & Technical Achievements
Improve the quantity and quality of seed proteins and proteins from vegetative plant tissues.
Engineering DNA | Biomolecular Engineering | Host Engineering | Data Science |
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Increase expression of proteins with desired amino acid content by introducing engineered genetic parts (including promoters and peptide tags). Modulate seed-specific pathway genes to enhance protein accumulation in seed without impacting germination. |
Design novel proteins that incorporate important amino acids that can be expressed in seed or improve protein solubility. De novo, model-based creation of proteins with high nutritional value. |
Introduce proteins of high nutritional value into plant tissue. |
Model protein and oil flux in the seed to ensure optimal accumulation. Produce better models of important proteins to understand where protein quality can be improved. |
Increase bioavailability of proteins through engineering structure, amino acid composition, and removing anti-nutritive factors.
Engineering DNA | Biomolecular Engineering | Host Engineering | Data Science |
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Edit plant protein sequences to increase digestibility through changes to structure and amino acid content or reduce expression of anti-nutritive factors. |
Design new proteins to increase content of rare or needed amino acids and/or enhanced solubility. Eliminate aspects of pathways that produce anti-nutritive factors that do not impact plant growth. |
Introduce or modulate pathways and feedback loops that play a role in protein degradation and amino acid scavenging. |
Enable (increased) production of beneficial fatty acids and digestible fiber in staple crops.
Engineering DNA | Biomolecular Engineering | Host Engineering | Data Science |
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Knockout genes/pathways that divert carbon away from fatty acid (FA) production in plants. Reduce lignin biosynthesis in crops. |
Improve enzymatic properties of proteins involved in conversion of FAs to long-chain polyunsaturated fatty acids (LC-PUFAs) to increase production and accumulation in plants. Develop more effective enzymes that can reduce lignin content in crops. |
Introduce pathways or novel enzymes from heterologous systems that are missing in plants to convert FAs into omega-3 or omega-6 LC-PUFAs. Integrate and/or replace poorly perfoming or less desireable pathway enzymes. Overexpress lignin-degradation enzyme(s) under an inducible promoter. |