Develop tools/methods for editing the chromosomes of microbes resident in seawater.

Biosynthetic pathways active in halophilic/hypersaline conditions.

Develop robust genome engineering for halophiles (e.g., Vibrio natriegens).

Engineer resistance to phage/toxins.

Strict biocontainment strategies for potential engineered organisms deployment.

Modeling of organism fitness in seawater.

Develop tools/methods for editing the chromosomes of microbes resident in wastewater treatment facilities.

Engineer enzymes to convert fats in wastewater to hydrocarbons.

Engineer resistance to phage/toxins.

Strict biocontainment strategies for potential engineered organisms deployment.

Engineer microbes for ammonium oxidation, denitrification, and polyphosphate accumulation.

Model competition of microbes in wastewater treatment facilities to predict survival of engineered organisms.