Efficient CRISPR systems.

Methods for efficiently editing organelle genomes.

Engineer enzymes/pathways for production of hydrocarbons, including decarboxylating/decarbonylating enzymes, cofactor changes on enzymes, and new pathways/enzymes that conserve carbon/energy.

Engineer transporters to facilitate fuel export from the cell.

Develop efficient pathways for production of liquid transportation fuels from metabolic intermediates.

Develop tools to enable engineering of carbon concentration/fixation pathways in CO2 fixers.

Large-scale knockout of unnecessary pathways.

Development of carbon transport and concentration mechanisms.

Organelle synthesis.

Metabolic flux analysis of engineered organisms.

Models of microbes in bioreactors to predict performance.

Technoeconomic and life cycle analysis models to determine sustainability of energy production.

BioCAD models for designing gene expression.

Efficient CRISPR systems.

Methods for efficiently editing organelle genomes.

Synthesize and transform genomes of organelles.

Light energy conversion: engineer microbes and algae to more efficiently convert solar light to carbon/ATP.

Light capture: expand the range of solar spectrum wavelengths that can be captured by photosynthesis.

Improve CO₂ fixation by reducing 2-phosphoglycolate produced by RuBISCO O₂ fixation.

Engineer enzymes/pathways for production of hydrocarbons, including decarboxylating/decarbonylating enzymes, cofactor changes on enzymes, and new pathways/enzymes that conserve carbon/energy.

Engineer transporters to facilitate fuel export from the cell.

Develop tools for controlling gene expression in photosynthetic microbes.

Develop efficient pathways for production of liquid transportation fuels from metabolic intermediates.

Development of carbon transport and concentration mechanisms.

Technoeconomic and life cycle analysis models to determine sustainability of energy production.

BioCAD models for designing gene expression.

Metabolic flux analysis of engineered organisms.

Models of microbes in photobioreactors to predict performance.

Efficient CRISPR systems.

Synthesize large clusters of genes that encode metabolic pathways for various products (including fuels, commodity chemicals, and specialty chemicals) ready to be transformed into any microbial host.

Recombinant expression of particulate and soluble methane monoxygenase (MMO) and demonstrate in vivo activity in a heterologous host.

Improve catalytic activity of MMO in native and heterologous hosts.

Engineer enzymes/pathways for production of hydrocarbons, including decarboxylating/decarbonylating enzymes, cofactor changes on enzymes, and new pathways/enzymes that conserve carbon/energy.

Engineer transporters to facilitate fuel export from the cell.

Growth on alternative carbon sources to allow systematic genetic screening of critical enzymes for methane utilization.

More efficient and rapid transformation methods.

Large-scale knockout of unnecessary pathways.

Develop efficient pathways for production of liquid transportation fuels from metabolic intermediates.

Metabolic flux analysis of engineered organisms.

Models of microbes in bioreactors to predict performance.

Technoeconomic and life cycle analysis models to determine sustainability of energy production.

BioCAD models for designing gene expression.

Efficient CRISPR systems.

Methods for efficiently editing organelle genomes.

Synthesize and transform genomes of organelles.

Light energy conversion: engineer microbes and algae to more efficiently convert solar light to carbon/ATP.

Light capture: expand the range of solar spectrum wavelengths that can be captured by photosynthesis.

Improve CO₂ fixation by reducing 2-phosphoglycolate produced by RuBISCO O₂ fixation.

Engineer enzymes/pathways for production of hydrocarbons, including decarboxylating/decarbonylating enzymes, cofactor
changes on enzymes, and new pathways/enzymes that conserve carbon/energy.

Engineer transporters to facilitate fuel export from the cell.

Engineer enzymes/pathways for production of hydrogen (via water splitting).

Develop tools for controlling gene expression in photosynthetic microbes.

Develop efficient pathways for production of liquid transportation fuels from metabolic intermediates.

Development of carbon transport and concentration mechanisms.

Technoeconomic and life cycle analysis models to determine sustainability of energy production.

BioCAD models for designing gene expression.

Metabolic flux analysis of engineered organisms.

Models of microbes in photobioreactors to predict performance.